Histamine stimulates human microglia to alter cellular prion protein expression via the HRH2 histamine receptor.

ABSTRACT: Although the cellular prion protein (PrP^C) has been evolutionarily conserved, the role of this protein remains elusive. Recent evidence indicates that PrP^C may be involved in neuroinflammation and the immune response in the brain, and its expression may be modified via various mechanisms. Histamine is a proinflammatory mediator and neurotransmitter that stimulates numerous cells via interactions with histamine receptors 1-4 (HRH1-4). Since microglia are the innate immune cells of the central nervous system, we hypothesized that histamine-induced stimulation regulates the expression of PrP^C in human-derived microglia. The human microglial clone 3 (HMC3) cell line was treated with histamine, and intracellular calcium levels were measured via a calcium flux as

SUBMITTER: Pehar M 

PROVIDER: S-EPMC11513956 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature