Hyperspectral oblique plane microscopy enables spontaneous, label-free imaging of biological dynamic processes in live animals.

ABSTRACT: Spontaneous Raman imaging has emerged as powerful label-free technique for investigating the molecular composition of medicines and biological specimens. Although Raman imaging can facilitate understanding of complex biological phenomena in vivo, current imaging modalities are limited in speed and sample compatibility. Here, we introduce a single-objective line-scanning light-sheet microscope, named [Formula: see text]-OPM, which records Raman images on a timescale of minutes to seconds. To demonstrate its function, we use [Formula: see text]-OPM to map and identify microplastic particles based on their Raman spectral characteristics. In live zebrafish embryos, we show that [Formula: see text]-OPM can capture wound dynamics at five-minute intervals, revealing rapid changes in cellular and

SUBMITTER: Guo K 

PROVIDER: S-EPMC11513980 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature