Development of a novel immunocompetent murine tumor model for urothelial carcinoma using in vivo electroporation.

ABSTRACT: A lack of advanced preclinical mouse tumor models impedes the progress in urothelial carcinoma research. We present here a novel fast, robust, reliable, and highly reproducible model for the genetic induction of bladder cancer in immunocompetent mice. Different sets of oncogenic transposons (Cmyc, Kras) and Cre drivers were transfected into the murine bladder wall of two different genetic backgrounds (Trp53^fl/fl and Braf^V600E, Pten^fl/fl, Ctnnb1^exon3-fl/fl). Transfection was carried out using in vivo electroporation of the bladder after surgical exploration and transmural or transurethral intravesical plasmid injection. Up to 100% of animals developed urothelial carcinomas of the bladder. Time to tumor onset ranged from 16 to 97 days with a median

SUBMITTER: Soleder S 

PROVIDER: S-EPMC11514157 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature