3'UTR of tobacco vein mottling virus regulates downstream GFP expression and changes in host gene expression.

ABSTRACT:

Introduction

Tobacco vein mottling virus (TVMV) is a member of the family Potyviridae. The 3' untranslated region (3'UTR) of viral genomic RNA has been reported to significantly impact viral infection. Nevertheless, the role of the TVMV 3'UTR during viral infection remains unknown.

Methods

Here, a 3'UTR-GFP expression vector was transiently expressed in Nicotiana benthamiana, in which the 3'UTR of TVMV was introduced upstream of the green fluorescent protein (GFP) gene. Transcriptome sequencing was performed to analyze the genes associated with plant resistance. The effect of the TVMV 3'UTR on GFP expression was studied using an Agrobacterium-mediated transient expression assay, revealing that the TVMV 3'UTR significantly inhibited GFP expression. Transc

SUBMITTER: Sun Z 

PROVIDER: S-EPMC11514416 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature