A CRISPR-dCas13 RNA-editing tool to study alternative splicing.

ABSTRACT: Alternative splicing allows multiple transcripts to be generated from the same gene to diversify the protein repertoire and gain new functions despite a limited coding genome. It can impact a wide spectrum of biological processes, including disease. However, its significance has long been underestimated due to limitations in dissecting the precise role of each splicing isoform in a physiological context. Furthermore, identifying key regulatory elements to correct deleterious splicing isoforms has proven equally challenging, increasing the difficulty of tackling the role of alternative splicing in cell biology. In this work, we take advantage of dCasRx, a catalytically inactive RNA targeting CRISPR-dCas13 ortholog, to efficiently switch alternative splicing patterns of endogenous transcript

SUBMITTER: Nunez-Alvarez Y 

PROVIDER: S-EPMC11514487 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature