α-Synuclein disrupts microglial autophagy through STAT1-dependent suppression of Ulk1 transcription.

ABSTRACT:

Background

Autophagy dysfunction in glial cells is implicated in the pathogenesis of Parkinson's disease (PD). The previous study reported that α-synuclein (α-Syn) disrupted autophagy in cultured microglia. However, the mechanism of microglial autophagy dysregulation is poorly understood.

Methods

Two α-Syn-based PD models were generated via AAV-mediated α-Syn delivery into the mouse substantia nigra and striatal α-Syn preformed fibril (PFF) injection. The levels of microglial UNC-51-like kinase 1 (Ulk1) and other autophagy-related genes in vitro and in PD mice, as well as in the peripheral blood mononuclear cells of PD patients and healthy controls, were determined via quantitative PCR, western blotting and immunostaining. The regulatory effect of signal transducer and activ

SUBMITTER: Pei CS 

PROVIDER: S-EPMC11515151 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature