Amphiregulin promotes activated regulatory T cell-suppressive function via the AREG/EGFR pathway in laryngeal squamous cell carcinoma.

ABSTRACT:

Background

Activated regulatory T cells (aTregs) play a vital role in promoting a tumor immunosuppressive microenvironment in laryngeal squamous cell carcinoma (LSCC). However, the regulatory factors that induce the generation of aTregs are not clear. Herein, we investigated the effect of amphiregulin (AREG) on the production of aTregs in the tumor microenvironment of LSCC.

Methods

Immunohistochemical (IHC) analysis was conducted to examine the expression of AREG and FOXP3, and their association with clinical parameters and patient outcomes was demonstrated. The expression level of EGFRs in three functional subsets of Tregs was assessed, and the induction of CD4⁺ T cells into aTregs in the presence or absence of AREG or Gefitinib was analyzed using flow cytometry.

SUBMITTER: Li H 

PROVIDER: S-EPMC11515249 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature