A single-stranded based library preparation method for virome characterization.

ABSTRACT:

Background

The gut virome is an integral component of the gut microbiome, playing a crucial role in maintaining gut health. However, accurately depicting the entire gut virome is challenging due to the inherent diversity of genome types (dsDNA, ssDNA, dsRNA, and ssRNA) and topologies (linear, circular, or fragments), with subsequently biases associated with current sequencing library preparation methods. To overcome these problems and improve reproducibility and comparability across studies, universal or standardized virome sequencing library construction methods are highly needed in the gut virome study.

Results

We repurposed the ligation-based single-stranded library (SSLR) preparation method for virome studies. We demonstrate that the SSLR method exhibits exceptional effi

SUBMITTER: Zhai X 

PROVIDER: S-EPMC11515303 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature