Comparison of different diagnostic protocols for the detection of Toxocara spp. in faecal samples of cats and dogs.

ABSTRACT:

Background

Toxocara canis and Toxocara cati are parasitic nematodes that occur worldwide. As embryonated Toxocara spp. eggs in the environment pose a zoonotic risk, especially for children, optimal diagnostic approaches are necessary for effective disease response and management, including surveillance. However, little is known about the performance of different diagnostic protocols for detecting Toxocara spp. in the faeces of cats and dogs, hampering movement towards an optimal diagnostic process. This study aimed to compare detection methods, including a newly developed sequential sieving protocol (SF-SSV) and a high-throughput multiplex qPCR-based method to facilitate epidemiological studies.

Methods

Species-specific Toxocara spp. egg suspensions and canine and feline fae

SUBMITTER: Winterfeld DT 

PROVIDER: S-EPMC11515329 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature