RNA-seq validation of microRNA expression signatures for precision melanoma diagnosis and prognostic stratification.

ABSTRACT:

Background

New diagnostic tools are needed to improve the diagnosis and risk stratification of cutaneous melanoma. Disease-specific microRNA signatures have been previously described via NanoString profiling of solid biopsy tissue and plasma. This study validated these signatures via next-generation sequencing technology and compared their performance against clinical metrics and other published melanoma signatures.

Methods

RNA from 64 plasma and 60 FFPE biopsy samples from individuals with invasive melanoma or related benign/control phenotypes was extracted and enriched for microRNA. RNA sequencing was performed to compute MEL38/MEL12 signature scores. The results were evaluated with published NanoString and RNA sequencing datasets, comprising 548 solid tissue samples and 2

SUBMITTER: Love CG 

PROVIDER: S-EPMC11515382 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature