SPOT: spatial proteomics through on-site tissue-protein-labeling.

ABSTRACT:

Background

Spatial proteomics seeks to understand the spatial organization of proteins in tissues or at different subcellular localization in their native environment. However, capturing the spatial organization of proteins is challenging. Here, we present an innovative approach termed Spatial Proteomics through On-site Tissue-protein-labeling (SPOT), which combines the direct labeling of tissue proteins in situ on a slide and quantitative mass spectrometry for the profiling of spatially-resolved proteomics.

Materials and methods

Efficacy of direct TMT labeling was investigated using seven types of sagittal mouse brain slides, including frozen tissues without staining, formalin-fixed paraffin-embedded (FFPE) tissues without staining, deparaffinized FFPE tissues, deparaffiniz

SUBMITTER: Xu Y 

PROVIDER: S-EPMC11515502 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature