Age, sex, and cell type-resolved hypothalamic gene expression across the pubertal transition in mice.

ABSTRACT:

Background

The hypothalamus plays a central role in regulating puberty. However, our knowledge of the postnatal gene regulatory networks that control the pubertal transition in males and females is incomplete. Here, we investigate the age-, sex- and cell-type-specific gene regulation in the hypothalamus across the pubertal transition.

Methods

We used RNA-seq to profile hypothalamic gene expression in male and female mice at five time points spanning the onset of puberty (postnatal days (PD) 12, 22, 27, 32, and 37). By combining this data with hypothalamic single nuclei RNA-seq data from pre- and postpubertal mice, we assigned gene expression changes to their most likely cell types of origin. In our colony, pubertal onset occurs earlier in male mice, allowing us to focus on g

SUBMITTER: Sokolowski DJ 

PROVIDER: S-EPMC11515584 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature