Engineering Escherichia coli via introduction of the isopentenol utilization pathway to effectively produce geranyllinalool.

ABSTRACT:

Background

Geranyllinalool, a natural diterpenoid found in plants, has a floral and woody aroma, making it valuable in flavors and fragrances. Currently, its synthesis primarily depends on chemical methods, which are environmentally harmful and economically unsustainable. Microbial synthesis through metabolic engineering has shown potential for producing geranyllinalool. However, achieving efficient synthesis remains challenging owing to the limited availability of terpenoid precursors in microorganisms. Thus, an artificial isopentenol utilization pathway (IUP) was constructed and introduced in Escherichia coli to enhance precursor availability and further improve terpenoid synthesis.

Results

We first constructed an artificial IUP in E. coli to enhance the supply of precurso

SUBMITTER: Chang J 

PROVIDER: S-EPMC11515624 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature