Identification of hub genes through integrated single-cell and microarray transcriptome analysis in osteoarthritic meniscus.

ABSTRACT:

Background

Osteoarthritis (OA) is marked by the progressive degradation of joint cartilage and subchondral bone. The precise molecular mechanisms driving meniscus deterioration in OA, especially at the single-cell level, remain poorly understood.

Method

We analyzed two datasets from the GEO database, GSE220243 and GSE98918, focusing on meniscus tissue sequencing data from OA and non-OA patients. The standard Seurat procedure was employed to process single-cell data and identify differentially expressed genes (DEGs). Immune cell infiltration was assessed using the Microenvironment Cell Populations (MCP) counter and CIBERSORT algorithms. For the microarray data, DEGs were identified with the limma package, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEG

SUBMITTER: Shen Y 

PROVIDER: S-EPMC11515729 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature