Transcriptomic analysis of cellular senescence induced by ectopic expression of ATF6α in human breast cancer cells.

ABSTRACT:

Background

The transcriptomic profile of cellular senescence is strongly associated with distinct cell types, the specific stressors triggering senescence, and temporal progression through senescence stages. This implies the potential necessity of conducting separate investigations for each cell type and a stressor inducing senescence. To elucidate the molecular mechanism that drives endoplasmic reticulum (ER) stress-induced cellular senescence in MCF-7 breast cancer cells, with a particular emphasis on the ATF6α branch of the unfolded protein response. We conducted transcriptomic analysis on MCF-7 cells by ectopic expression of ATF6α.

Methods

Transcriptomic sequencing was conducted on MCF-7 cells at 6 and 9 hours post senescence induction through ATF6α ectopic expression. C

SUBMITTER: Kim JW 

PROVIDER: S-EPMC11515977 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature