Transition characteristics and thermodynamic analysis of DNA duplex formation: a quantitative consideration for the extent of duplex association.

ABSTRACT: Transition characteristics and thermodynamic properties of the single-stranded self-transition and the double-stranded association were investigated and analyzed for 9-, 15- and 21-bp non-self-complementary DNA sequences. The multiple transition processes for the single-stranded self-transition and the double-stranded association were further put forth. The experimental results confirmed that the double-stranded association transition was generally imperfect and the thermodynamic properties of the single-stranded self-transition would exert an influence on a duplex formation. Combining ultraviolet melting experiments in various molar ratios, the extent of duplex association was estimated for three double-stranded DNAs. In our experimental range, the extent of duplex association decreases w

SUBMITTER: Wu P 

PROVIDER: S-EPMC115173 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature