Molecular characterization of plasmid pBM300 from Bacillus megaterium QM B1551.

ABSTRACT: Strain QM B1551 of Bacillus megaterium contains seven compatible plasmids: two small rolling circle plasmids and five theta-replicating plasmids with cross-hybridizing replicons. To expand our understanding of these plasmids, the replicon region (6.7 kb) from pBM300 was cloned, sequenced, and functionally characterized. Sequence analysis showed that the replication protein (RepM300) was highly homologous to two other plasmid Rep proteins of the same strain but to no other known proteins. Furthermore, the location of the replication origin was within the RepM300 coding region, and the origin contained three 12-base direct repeats. Deletion analysis of the replicon confirmed the role of the Rep protein and showed that open reading frame 2 (ORF2) was required for stability. However, the prote

SUBMITTER: Kunnimalaiyaan M 

PROVIDER: S-EPMC1151835 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature