Diverse new plasmid structures and antimicrobial resistance in strains isolated from perianal abscess patients.

ABSTRACT:

Introduction

Plasmids, the most important and versatile bacterial extrachromosomal DNA Molecules, has have been a center central topic for bacterial genetics and biology. However, the inability of short-read high-throughput sequencing methods to reliably assemble plasmids makes it difficult to investigate the diversity of plasmid structures and functions.

Methods

In this work, we used the long-read Nanopore sequencing method to address this issue, by producing high quality whole genome sequences of 33 bacterial strains from 11 perianal abscess-suffering patients.

Results and discussion

Successful high quality assemblies were generated with this method, including 20 perfect assemblies out of 33 genomes. A total of 47 plasmids were identified from the bacterial strains,

SUBMITTER: Xu Z 

PROVIDER: S-EPMC11518769 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature