Multiple copies of the 16S rRNA gene in Nocardia nova isolates and implications for sequence-based identification procedures.

ABSTRACT: Molecular investigation of two Nocardia patient isolates showed unusual restriction fragment length polymorphism patterns with restriction endonuclease assays (REA) using an amplified portion of the 16S rRNA gene. Patterns typical of Nocardia nova were obtained with REA of an amplified portion of the 65-kDa heat shock protein gene. Subsequent sequence analysis of the 16S rRNA gene regions of these isolates showed the presence of ambiguous bases within an expected restriction endonuclease recognition site which were not able to be resolved on repeat testing. Cloning of amplified regions of the 16S rRNA genes and subsequent sequencing of the resulting clones from the two patient isolates showed three different 16S rRNA gene sequences which corresponded to sequences found in N. nova, a molecu

SUBMITTER: Conville PS 

PROVIDER: S-EPMC1151890 | biostudies-literature | 2005 Jun

REPOSITORIES: biostudies-literature