Sodium citrate targeting Ca²⁺/CAMKK2 pathway exhibits anti-tumor activity through inducing apoptosis and ferroptosis in ovarian cancer.

ABSTRACT:

Introduction

Ovarian cancer (OC) is known for its high mortality rate. Although sodium citrate has anti-tumor effects in various cancers, its effect and mechanism in OC remain unclear.

Objectives

To analyze the inhibitory effect of sodium citrate on ovarian cancer cells and the underlying mechanism.

Methods

Cell apoptosis was examined by TUNEL staining, flow cytometry, and ferroptosis was examined intracellular Fe²⁺, MDA, LPO assays, respectively. Cell metabolism was examined by OCR and ECAR measurements. Immunoblotting and immunoprecipitation were used to elucidate the mechanism.

Results

This study suggested that sodium citrate not only promoted ovarian cancer cell apoptosis but also triggeredferroptosis, manifested as elevated levels of Fe^2+</

SUBMITTER: Wu Y 

PROVIDER: S-EPMC11518946 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature