NanoString nCounter-Based Assay for Detection of Fusion-Associated Salivary Gland Tumors.

ABSTRACT:

Purpose

Salivary gland tumors include numerous subtypes that vary from benign to highly aggressive, with many showing overlapping histopathological features that can make diagnosis challenging. Most subtypes express driver fusion genes that are tumor specific, and detection of such fusions is useful for differentiating amongst specific diagnoses, determining appropriate tumor grading, and guiding effective treatment. Currently, fusions can be detected by FISH, RT-PCR or through next-generation sequencing approaches, all of which are highly effective methodologies but can be costly or time consuming.

Methods

We developed a rapid NanoString nCounter platform-based assay to detect salivary gland tumor fusions using a combination of fusion junction-specific probes and an approac

SUBMITTER: Goytain A 

PROVIDER: S-EPMC11519273 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature