5'-tRNA^Gly(GCC) halves generated by IRE1α are linked to the ER stress response.

ABSTRACT: Transfer RNA halves (tRHs) have various biological functions. However, the biogenesis of specific 5'-tRHs under certain conditions remains unknown. Here, we report that inositol-requiring enzyme 1α (IRE1α) cleaves the anticodon stem-loop region of tRNA^Gly(GCC) to produce 5'-tRHs (5'-tRH-Gly^GCC) with highly selective target discrimination upon endoplasmic reticulum (ER) stress. Levels of 5'-tRH-Gly^GCC positively affect cancer cell proliferation and modulate mRNA isoform biogenesis both in vitro and in vivo; these effects require co-expression of two nuclear ribonucleoproteins, HNRNPM and HNRNPH2, which we identify as binding proteins of 5'-tRH-Gly^GCC. In addition, under ER stress in vivo, we observe simultaneous induction of IRE1α and 5'-tRH-Gly

SUBMITTER: Jin H 

PROVIDER: S-EPMC11519470 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature