Loss of EMI1 compromises chromosome stability and is associated with cellular transformation in colonic epithelial cell contexts.

ABSTRACT:

Background

Colorectal cancer (CRC) is still a leading cause of cancer deaths worldwide. Thus, identifying the aberrant genes and proteins underlying disease pathogenesis is critical to improve early detection methods and develop novel therapeutic strategies. Chromosome instability (CIN), or ongoing changes in chromosome complements, is a predominant form of genome instability. It is a driver of genetic heterogeneity found in ~85% of CRCs. Although CIN contributes to CRC pathogenesis, the molecular determinants underlying CIN remain poorly understood. Recently, EMI1, an F-box protein, was identified as a candidate CIN gene. In this study, we sought to determine the impact reduced EMI1 expression has on CIN and cellular transformation.

Methods

Coupling siRNA-based silencing an

SUBMITTER: Campos Gudino R 

PROVIDER: S-EPMC11519589 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature