A novel rat lung transplantation model using venoarterial extracorporeal membrane oxygenation support.

ABSTRACT:

Objectives

Extracorporeal membrane oxygenation (ECMO) has become an important life support technique during lung transplantation. We aimed to develop a rat model for lung transplantation using venoarterial (VA) ECMO support.

Methods

Adult male Sprague-Dawley rats weighing 400 to 450 g were used in this study. ECMO circuits were created by obtaining venous access from the femoral vein with subsequent extracorporeal oxygen exchange, which was then returned to the circulatory system through the left carotid artery (ie, VA-ECMO). Simultaneously, the donor lungs were retrieved and immersed in cold, low-potassium dextran lung preservation solution. Orthotopic left lung transplantation supported by VA-ECMO was performed. Thereafter, a respiratory failure rat model was constructed u

SUBMITTER: Yang X 

PROVIDER: S-EPMC11519749 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature