Luminescent Ln(III)-Metallopeptide Sensors for Monitoring <i>Pseudomonas aeruginosa</i> Elastase B Activity in Complex Biological Media.

ABSTRACT: The detection and monitoring of Pseudomonas aeruginosa and its virulence factors, such as the LasB protease, are crucial for managing bacterial infections. Traditional fluorescent sensors for this protease face limitations in bacterial cultures due to interference from pigments like pyoverdine secreted by this opportunistic pathogen. We report here a Ln(III)-metallopeptide that combines a DO3A-Ln(III) complex and a sensitizing unit via a short peptide sequence as a simple, tunable, and selective probe for detecting P. aeruginosa's LasB. The probe's luminescence switches off in the presence of P. aeruginosa's secretome due to LasB cleavage but remains stable in other bacterial environments, such as non-LasB-secreting P. aeruginosa strains or E. coli cultur

SUBMITTER: Sanchez-Fernandez R 

PROVIDER: S-EPMC11519908 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature