Novel Approach for Lifetime-Proportional Luminescence Imaging Using Frame Straddling.

ABSTRACT: Optode-based chemical imaging is a rapidly evolving field that has substantially enhanced our understanding of the role of microenvironments and chemical gradients in biogeochemistry, microbial ecology, and biomedical sciences. Progress in sensor chemistry has resulted in a broadened spectrum of analytes, alongside enhancements in sensor performance (e.g., sensitivity, brightness, and photostability). However, existing imaging techniques are often costly, challenging to implement, and limited in their recording speed. Here we use the "frame-straddling" technique, originally developed for particle image velocimetry for imaging the O₂-dependent, integrated luminescence decay of optical O₂ sensor materials. The method synchronizes short excitation pulses and camera expos

SUBMITTER: Ahmerkamp S 

PROVIDER: S-EPMC11519917 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature