Genistein inhibited endocytosis and fibrogenesis in keloid via CTGF signaling pathways.

ABSTRACT:

Background

This study aimed to evaluate soy isoflavones' effect and potential use-specifically genistein-in treating human keloid fibroblast cells (KFs) and in a keloid tissue culture model.

Methods

To investigate the effects of genistein on keloid, a wound-healing assay was performed to detect cell migration. Flow cytometry was used to measure apoptosis. Western blotting and immunofluorescence staining were performed to detect the expression of target proteins. KF tissues were isolated, cultured, and divided into the control, silenced connective tissue growth factor (CTGF) proteins, and shNC (negative control) groups.

Results

Genistein suppressed cell proliferation and migration, triggering the cell cycle at the G2/M phase and increasing the expression of p53 dose-de

SUBMITTER: Lu CT 

PROVIDER: S-EPMC11520065 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature