Adhesion of <i>Crithidia fasciculata</i> promotes a rapid change in developmental fate driven by cAMP signaling.

ABSTRACT: Trypanosomatids are single-celled parasites responsible for human and animal disease. Typically, colonization of an insect host is required for transmission. Stable attachment of parasites to insect tissues via their single flagellum coincides with differentiation and morphological changes. Although attachment is a conserved stage in trypanosomatid life cycles, the molecular mechanisms are not well understood. To study this process, we elaborate upon an in vitro model in which the swimming form of the trypanosomatid Crithidia fasciculata rapidly differentiates following adhesion to artificial substrates. Live imaging of cells transitioning from swimming to attached shows parasites undergoing a defined sequence of events, including an initial adhesion near the base of t

SUBMITTER: Denecke S 

PROVIDER: S-EPMC11520290 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature