Antibacterial effectors in Dictyostelium discoideum: specific activity against different bacterial species.

ABSTRACT: Dictyostelium discoideum is a phagocytic amoeba continuously eating, killing, and digesting bacteria. Previous studies have detected in D. discoideum cell extracts a bacteriolytic activity effective against Klebsiella pneumoniae bacteria. In this study, we characterized bacteriolytic activities found in D. discoideum cell extracts against five different bacteria (K. pneumoniae, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus subtilis). We first analyzed the bacteriolytic activity against these five bacteria in parallel over a range of pH values. We then measured the remaining bacteriolytic activity in D. discoideum kil1 and modA knockout mutants. We also performed partial fractionatio

SUBMITTER: Munoz-Ruiz R 

PROVIDER: S-EPMC11520349 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature