A tumorigenicity evaluation platform for cell therapies based on brain organoids.

ABSTRACT:

Background

Tumorigenicity represents a critical challenge in stem cell-based therapies requiring rigorous monitoring. Conventional approaches for tumorigenicity evaluation are based on animal models and have numerous limitations. Brain organoids, which recapitulate the structural and functional complexity of the human brain, have been widely used in neuroscience research. However, the capacity of brain organoids for tumorigenicity evaluation needs to be further elucidated.

Methods

A cerebral organoid model produced from human pluripotent stem cells (hPSCs) was employed. Meanwhile, to enhance the detection sensitivity for potential tumorigenic cells, we created a glioblastoma-like organoid (GBM organoid) model from TP53^-/-/PTEN^-/- hPSCs to provide a tumo

SUBMITTER: Xue J 

PROVIDER: S-EPMC11520457 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature