Asiatic acid inhibits HBV cccDNA transcription by promoting HBx degradation.

ABSTRACT:

Background

Hepatitis B virus (HBV) infection is a persistent global public health problem, and curing for chronic hepatitis B (CHB) through the application of existing antiviral drugs is beset by numerous challenges. The viral protein HBx is a critical regulatory factor in the life cycle of HBV. Targeting HBx is a promising possibility for the development of novel therapeutic strategies.

Methods

The Nano-Glo^® HiBiT Lysis Detection System was used to screen the herbal monomer compound library for compounds that inhibit HBx expression. Western blotting was used to examine proteins expression. Southern blotting or Northern blotting were used to detect HBV DNA or HBV RNA. ELISA was performed to detect the HBsAg level. The effect of asiatic acid on HBV in vivo was inve

SUBMITTER: Li R 

PROVIDER: S-EPMC11520515 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature