Detecting complex infections in trypanosomatids using whole genome sequencing.

ABSTRACT:

Background

Trypanosomatid parasites are a group of protozoans that cause devastating diseases that disproportionately affect developing countries. These protozoans have developed several mechanisms for adaptation to survive in the mammalian host, such as extensive expansion of multigene families enrolled in host-parasite interaction, adaptation to invade and modulate host cells, and the presence of aneuploidy and polyploidy. Two mechanisms might result in "complex" isolates, with more than two haplotypes being present in a single sample: multiplicity of infections (MOI) and polyploidy. We have developed and validated a methodology to identify multiclonal infections and polyploidy using whole genome sequencing reads, based on fluctuations in allelic read depth in heterozygous positi

SUBMITTER: Reis-Cunha JL 

PROVIDER: S-EPMC11520695 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature