Circ-CAMTA1 regulated by Ca²⁺ influx inhibited pyruvate carboxylase activity and modulate T cell function in patients with systemic lupus erythematosus.

ABSTRACT:

Objectives

To investigate the roles of Ca²⁺ influx-regulated circular RNAs (circRNAs) in T cells from patients with systemic lupus erythematosus (SLE).

Methods

The expression profile of circRNAs in Jurkat cells, co-cultured with and without ionomycin, was analyzed by next-generation sequencing and validated using real-time polymerase chain reaction. The identified Ca²⁺ influx-regulated circRNAs were further examined in T cells from 42 patients with SLE and 23 healthy controls. The biological function of specific circRNA was investigated using transfection and RNA pull-down assay.

Results

After validation, we confirmed that the expression levels of circ-ERCC4, circ-NFATC2, circ-MYH10, circ-CAMTA1, circ-ASH1L, circ-SOCS7, and circ-ASAP1 were consis

SUBMITTER: Yu HC 

PROVIDER: S-EPMC11520813 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature