Establishment of a new method to isolate viable x-ray-sensitive cells from planarian by fluorescence-activated cell sorting.

ABSTRACT: Planarians show outstanding regenerative ability due to the proliferation of neoblasts. Hence the method to isolate planarian neoblasts is important to understand the regeneration process. In our previous study, we reported a method to isolate planarian neoblasts of Dugesia japonica using fluorescence-activated cell sorting (FACS). However, we have not yet succeeded in cultivating these cells even under in vivo conditions after transplantation into x-ray-irradiated planarians. This suggests that dissociated cells might enter apoptotic or necrotic states in the process of fluorescent dye staining and sorting. Here, we developed a new method to isolate viable neoblasts, which can proliferate in the x-ray-irradiated planarians. First, the toxicity of various fluorescence dyes was investigated

SUBMITTER: Ishida M 

PROVIDER: S-EPMC11520976 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature