Defining the molecular response to ischemia-reperfusion injury and remote ischemic preconditioning in human kidney transplantation.

ABSTRACT:

Background

Ischemia-reperfusion injury (IRI) inevitably occurs during kidney transplantation and extended ischemia is associated with delayed graft function and poor outcomes. Remote ischemic preconditioning (RIPC) is a simple, noninvasive procedure aimed at reducing IRI and improving graft function. Experimental studies have implicated the kynurenine pathway as a protective mechanism behind RIPC.

Methods

First, paired biopsies from 11 living kidney donors were analyzed to characterize the acute transcriptomic response to IRI. Second, 16 living kidney donors were subjected to either RIPC (n = 9) or no pretreatment (n = 7) to evaluate the impact of RIPC on the transcriptomic response to IRI. Finally, the effect of RIPC on plasma metabolites was analyzed in 49 healthy subjects

SUBMITTER: Nordstrom J 

PROVIDER: S-EPMC11521294 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature