MDM2 Is Essential to Maintain the Homeostasis of Epithelial Cells by Targeting p53.

ABSTRACT:

Introduction

MDM2 is known as the primary negative regulator of p53, and MDM2 promotes lung cancer fibrosis and lung injury through p53-dependent and p53-independent pathways. However, the mechanism by which MDM2 influences the pathogenesis of asthma is unknown. In this study, we investigated the function of MDM2 in lung epithelial cells in type 2 lung inflammation.

Methods

We used type II alveolar epithelial cell-specific heterozygous knockout of Mdm2 mice to validate its function. Then papain-induced asthma model was established, and changes in inflammation were observed by measuring immunohistochemistry and flow cytometry analysis.

Results

In this study, we knockdown the mouse Mdm2 gene in type 2 alveolar epithelial cells. We demonstrated that heterozygous Mdm2 gen

SUBMITTER: Wang S 

PROVIDER: S-EPMC11521410 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature