Impact of Extreme Prematurity, Chorioamnionitis, and Sepsis on Neonatal Monocyte Characteristics and Functions.

ABSTRACT:

Introduction

The innate branch of the immune system is important in early life, in particular for infants born preterm.

Methods

We performed a longitudinal analysis of the peripheral monocyte compartment in extremely preterm children from a randomized, placebo-controlled study of probiotic supplementation. PBMCs and fecal samples were collected at several timepoints during the first months of life. Monocyte characteristics were analyzed by flow cytometry, and LPS-stimulated PBMC culture supernatants were analyzed by Luminex or ELISA. Plasma cytokines and gut microbiota composition were analyzed by ELISA and 16S rRNA-sequencing, respectively.

Results

The extremely preterm infants had persistent alterations in their monocyte characteristics that were further aggravated

SUBMITTER: Qazi KR 

PROVIDER: S-EPMC11521501 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature