Discovery, characterization, and synthetic potential of two novel bacterial aryl-alcohol oxidases.

ABSTRACT: The search for novel synthetic tools to prepare industrial chemicals in a safer and greener manner is a continuing challenge in synthetic chemistry. In this manuscript, we report the discovery, characterization, and synthetic potential of two novel aryl-alcohol oxidases from bacteria which are able to oxidize a variety of aliphatic and aromatic alcohols with efficiencies up to 4970 min^-1 mM^-1. Both enzymes have shown a reasonable thermostability (thermal melting temperature values of 50.9 and 48.6 °C for ShAAO and SdAAO, respectively). Crystal structures revealed an unusual wide-open entrance to the active-site pockets compared to that previously described for traditional fungal aryl-alcohol oxidases, which could be associated with differences observed in substrate sc

SUBMITTER: Cinca-Fernando P 

PROVIDER: S-EPMC11522167 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature