Acanthamoeba polyphaga de novo transcriptome and its dynamics during Mimivirus infection.

ABSTRACT: Mimivirus bradfordmassiliense (Mimivirus) is a giant virus that infects Acanthamoeba species - opportunistic human pathogens. Long- and short-read sequencing were used to generate a de novo transcriptome of the host and followed the dynamics of both host and virus transcriptomes over the course of infection. The assembled transcriptome of the host included 22,604 transcripts and 13,043 genes, with N50 = 2,372 nucleotides. Functional enrichment analysis revealed major changes in the host transcriptome, namely, enrichment in downregulated genes associated with cytoskeleton homeostasis and DNA replication, repair, and nucleotide synthesis. These modulations, together with those implicated by other enriched processes, indicate cell cycle arrest, which was demonstrated experimentally. We also o

SUBMITTER: Bickels Nuri R 

PROVIDER: S-EPMC11522460 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature