A novel genetic strategy to enable rapid detection of rare non-native alleles.

ABSTRACT: Established invasive species represent one of the most harmful and challenging threats to native biodiversity, necessitating methods for Early Detection and Rapid Response. Cryptic invasions are particularly challenging and often require expensive and time-consuming molecular surveys which limits their usefulness for management. We present a novel application of the Fluidigm SNP-Type Assay to identify rare non-native alleles that significantly reduces the cost and time to generate diagnostic results. We demonstrate the efficacy of this method using experimental Fluidigm pools (99% accuracy) and sequence data (96% accuracy). We apply our novel methodology to an endangered population of California tiger salamanders in Sonoma County where two individual non-native tiger salamander hybrids hav

SUBMITTER: Cooper RD 

PROVIDER: S-EPMC11522522 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature