Exopeptidase combination enhances the degradation of isotopically labelled gluten immunogenic peptides in humans.

ABSTRACT:

Introduction

Celiac disease is a common autoimmune-like enteropathy caused by an aberrant response to incompletely digested dietary gluten. Gluten immunogenic peptides including the immunodominant 33-mer are thought to be resistant to proteolytic digestion by human gastrointestinal peptidases. We developed a novel enzyme therapy approach to support gluten peptide digestion using a combination of two tandem-acting exopeptidases, AMYNOPEP, that complement the intrinsic enzymatic activity of intestinal brush border enterocytes.

Methods

We evaluated the effects of AMYNOPEP supplementation on 33-mer degradation in vitro and in vivo. In a cross-over clinical study, healthy volunteers with no gastrointestinal disorders were given stable isotope (SI) labelled 33-mer pe

SUBMITTER: Mourabit S 

PROVIDER: S-EPMC11522800 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature