Digoxin and exercise effects on skeletal muscle Na⁺,K⁺-ATPase isoform gene expression in healthy humans.

ABSTRACT: In muscle, digoxin inhibits Na⁺,K⁺-ATPase (NKA) whereas acute exercise can increase NKA gene expression, consistent with training-induced increased NKA content. We investigated whether oral digoxin increased NKA isoform mRNA expression (qPCR) in muscle at rest, during and post-exercise in 10 healthy adults, who received digoxin (DIG, 0.25 mg per day) or placebo (CON) for 14 days, in a randomised, double-blind and cross-over design. Muscle was biopsied at rest, after cycling 20 min (10 min each at 33%, then 67% V̇O2peak${{\dot{V}}_{{{{\mathrm{O}}}_2}{\mathrm{peak}}}}$ ), then to fatigue at 90% V̇O2peak${{\dot{V}}_{{{{\mathrm{O}}}_2}{\mathrm{peak}}}}$ and 3 h post-exercise. No differences were found between DIG and CON for NKA α_1-3 or β_1-3 isoform

SUBMITTER: McKenna MJ 

PROVIDER: S-EPMC11522823 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature