Selectivity of Complex Coacervation in Multiprotein Mixtures.

ABSTRACT: Liquid-liquid phase separation of biomolecules is increasingly recognized as being relevant to various cellular functions, and complex coacervation of biomacromolecules, particularly proteins, is emerging as a key mechanism for this phenomenon. Complex coacervation is also being explored as a potential protein purification method due to its potential scalability, aqueous operation, and ability to produce a highly concentrated product. However, to date, most studies of complex coacervation have evaluated the phase behavior of a binary mixture of two oppositely charged macromolecules. Therefore, a comprehensive understanding of the phase behavior of complex biological mixtures is yet to be established. To address this, a panel of engineered proteins was designed to allow for quantitative ana

SUBMITTER: Ahn SY 

PROVIDER: S-EPMC11522905 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature