Fluorescent Water-Soluble Polycationic Chitosan Polymers as Markers for Biological 3D Imaging.

ABSTRACT: Over the last decades, various tissue-clearing techniques have broken the ground for the optical imaging of whole organs and whole-organisms, providing complete representative data sets of three-dimensional biological structures. Along with advancements in this field, the development of fluorescent markers for staining vessels and capillaries has offered insights into the complexity of vascular networks and their impact on disease progression. Here we describe the use of a modified water-soluble chitosan linked to cyanine dyes in combination with ethyl cinnamate-based optical tissue clearing for the 3D visualization of tissue vasculature in depth. The water-soluble fluorescent Chitosans have proven to be an optimal candidate for labeling both vessels and capillaries ex vivo thanks t

SUBMITTER: Vajpayee S 

PROVIDER: S-EPMC11522997 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature