Whole-genome sequencing-based analysis of Brucella species isolated from ruminants in various regions of Turkiye.

ABSTRACT:

Background

Brucellosis, a zoonotic disease in Türkiye, which has significant direct and indirect impacts on the healthcare system and livestock. This study, which aimed to investigate the differences among Brucella spp. isolates originating from different regions of Türkiye, for implications for public health and veterinary medicine.

Method

Twenty-one isolates from ruminants and two isolates from humans obtained from various regions of Türkiye were utilized in the study. The isolates were identified and biotyped using traditional microbiological procedures, and whole-genome sequencing (WGS) was performed. This was followed by single nucleotide polymorphism (SNP)--based phylogenetic analysis and WGS-based analysis of virulence and resistance genes. Additionally, phenotypic an

SUBMITTER: Otkun S 

PROVIDER: S-EPMC11524016 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature