Single cell atlas of kidney cancer endothelial cells reveals distinct expression profiles and phenotypes.

ABSTRACT:

Background

Tumor endothelial cells (TECs) represent the primary interface between the tumor microenvironment and circulating immune cells, however their phenotypes are incompletely understood in highly vascularized clear cell renal cell carcinoma (ccRCC).

Methods

We purified tumor and matched normal endothelial cells (NECs) from ccRCC specimens and performed single-cell RNA-sequencing to create a reference-quality atlas available as a searchable web resource for gene expression patterns. We established paired primary TECs and NECs cultures for ex vivo functional testing.

Results

TECs from multiple donors shared a common phenotype with increased expression of pathways related to extracellular matrix regulation, cell-cell communication, and insulin-like growth factor si

SUBMITTER: Xu Y 

PROVIDER: S-EPMC11524058 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature