Purification and membrane interactions of human KCNQ1_100-370 potassium ion channel.

ABSTRACT: KCNQ1 (Kv7.1 or KvLQT1) is a voltage-gated potassium ion channel that is involved in the ventricular repolarization following an action potential in the heart. It forms a complex with KCNE1 in the heart and is the pore forming subunit of slow delayed rectifier potassium current (I_ks). Mutations in KCNQ1, leading to a dysfunctional channel or loss of activity have been implicated in a cardiac disorder, long QT syndrome. In this study, we report the overexpression, purification, biochemical characterization of human KCNQ1_100-370, and lipid bilayer dynamics upon interaction with KCNQ1_100-370. The recombinant human KCNQ1 was expressed in Escherichia coli and purified into n-dodecylphosphocholine (DPC) micelles. The pu

SUBMITTER: Dixit G 

PROVIDER: S-EPMC11524546 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature