The zinc transporter Slc30a1 (ZnT1) in macrophages plays a protective role against attenuated Salmonella.

ABSTRACT: The zinc transporter Slc30a1 plays an essential role in maintaining cellular zinc homeostasis. Despite this, its functional role in macrophages remains largely unknown. Here, we examine the function of Slc30a1 in host defense using mice models infected with an attenuated stain of Salmonella enterica Typhimurium and primary macrophages infected with the attenuated Salmonella. Bulk transcriptome sequencing in primary macrophages identifies Slc30a1 as a candidate in response to Salmonella infection. Whole-mount immunofluorescence and confocal microscopy imaging of primary macrophage and spleen from Salmonella-infected Slc30a1^flag-EGFP mice demonstrate Slc30a1 expression is increased in infected macrophages with localization at the plasma membrane

SUBMITTER: Na-Phatthalung P 

PROVIDER: S-EPMC11524588 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature