Topical Platelet Exosomes Reduce Senescence Signaling in Human Skin: An Exploratory Prospective Trial.

ABSTRACT:

Background

Cellular senescence, an irreversible cell cycle arrest with secretory phenotype, is a hallmark of skin aging. Regenerative exosome-based approaches, such as topical human platelet extract (HPE), are emerging to target age-related skin dysfunction.

Objective

To evaluate the cellular and molecular effects of topical HPE for skin rejuvenation after 12 weeks of twice daily use.

Methods

Skin biopsies were obtained for histological evaluation of senescence markers, p16INK4a and p21CIP1/WAF1. Telomere-associated foci, coassociation of telomeres, and DNA damage marker, γH2AX, were assessed. RNA sequencing evaluated senescence associated secretory phenotype (SASP) and extracellular matrix pathways.

Results

p16INK4a and p21CIP1/WAF1 staining in senescent skin

SUBMITTER: Wyles SP 

PROVIDER: S-EPMC11524632 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature