Biomolecular condensates mediate bending and scission of endosome membranes.

ABSTRACT: Multivesicular bodies are key endosomal compartments implicated in cellular quality control through their degradation of membrane-bound cargo proteins^1-3. The ATP-consuming ESCRT protein machinery mediates the capture and engulfment of membrane-bound cargo proteins through invagination and scission of multivesicular-body membranes to form intraluminal vesicles^4,5. Here we report that the plant ESCRT component FREE1⁶ forms liquid-like condensates that associate with membranes to drive intraluminal vesicle formation. We use a minimal physical model, reconstitution experiments and in silico simulations to identify the dynamics of this process and describe intermediate morphologies of nascent intraluminal vesicles. Furthermore, we find that condensate-wetting-i

SUBMITTER: Wang Y 

PROVIDER: S-EPMC11525194 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature